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mitotracker deep red staining kit  (Keygen Biotech)

 
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    Keygen Biotech mitotracker deep red staining kit
    FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
    Mitotracker Deep Red Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitotracker deep red staining kit/product/Keygen Biotech
    Average 90 stars, based on 1 article reviews
    mitotracker deep red staining kit - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts"

    Article Title: Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-13860-9

    FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
    Figure Legend Snippet: FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).

    Techniques Used: Western Blot, Control, Lysis, Fluorescence, Positive Control, Staining, Membrane, Comparison



    Similar Products

    mitotracker deep red staining kit  (Keygen Biotech)
    90
    Keygen Biotech mitotracker deep red staining kit
    FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
    Mitotracker Deep Red Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitotracker deep red staining kit/product/Keygen Biotech
    Average 90 stars, based on 1 article reviews
    mitotracker deep red staining kit - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).

    Journal: Scientific Reports

    Article Title: Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts

    doi: 10.1038/s41598-017-13860-9

    Figure Lengend Snippet: FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).

    Article Snippet: The apoptosis rates, cell cycle distribution, mitochondrial membrane potential, ROS levels and mitochondrial mass were assessed with the Annexin V-PE/7-AAD staining kit (KeyGen Biotech, Nanjing, China), cell cycle analysis kit (Beyotime, Shanghai, China), JC-1 detection kit (KeyGen Biotech, Nanjing, China), ROS assay kit (Beyotime, Shanghai, China), and MitoTracker Deep Red staining kit (KeyGen Biotech, Nanjing, China), respectively.

    Techniques: Western Blot, Control, Lysis, Fluorescence, Positive Control, Staining, Membrane, Comparison